IN VITRO FUNCTIONAL PROPERTIES OF THE RAT BLADDER REGENERATED BY THE BLADDER ACELLULAR MATRIX GRAFT
Identifieur interne : 001963 ( Main/Exploration ); précédent : 001962; suivant : 001964IN VITRO FUNCTIONAL PROPERTIES OF THE RAT BLADDER REGENERATED BY THE BLADDER ACELLULAR MATRIX GRAFT
Auteurs : Hans J. Piechota [États-Unis] ; Stefan E. Dahms [États-Unis] ; Lora S. Nunes [États-Unis] ; Rajvir Dahiya [États-Unis] ; Tom F. Lue [États-Unis] ; Emil A. Tanagho [États-Unis]Source :
- The Journal of Urology [ 0022-5347 ] ; 1998.
English descriptors
- Teeft :
- Acellular, Adrenergic, Agonist, Atropine, Augmentation, Augmentation cystoplasty, Bamg, Biodegradable materials, Bladder, Bladder acellular matrix graft, Bladder reconstruction, Bladder stone formation, Bladder strips, Bladder substitution, Blood vessels, Carbachol, Carbachol stimulation, Cholinergic, Collagen content, Contractile, Contractile response, Contractile responses, Cystoplasty, Detrusor, Electrical field stimulation, Field stimulation, Functional innervation, Functional regeneration, Graft, Great variety, Higher dose, Histological, Host bladder, Host bladder response, Host bladder strips, Host bladder wall tissue, Immunohistochemical evaluation, Individual muscle strip, Isoproterenol, Krebs buffer solution, Lower line, Matrix, Maximum forces, Months postoperatively, Muscle bundles, Muscle strips, Nerve fibers, Norepinephrine, Normal micturition, Oxide pathway, Partial cystectomy, Peak contraction values, Peak contractions, Pharmacol, Previous studies, Protein gene product, Regenerated, Regeneration, Same extent, Seromuscular colocystoplasty, Sigma, Smooth muscle, Spontaneous muscle activity, Stock solutions, Stone formation, Subsequent administration, Tion, Tissue bath, Tissue bath experiments, Upper line, Urinary, Urinary bladder, Urinary tract, Urol, Urology, Valethamate, Valethamate bromide.
Abstract
Abstract: Purpose To assess the response of rat urinary bladder regenerated by the homologous bladder acellular matrix graft (BAMG) to in vitro electrical and pharmacologic stimuli.Materials and Methods In Sprague-Dawley rats, partial cystectomy (>50) was performed, followed by BAMG augmentation cystoplasty. After 4 months, organ bath studies of tissue strips in 10 were used to compare the contractility of the BAMG regenerates and the corresponding host detrusor smooth muscle.Results The BAMG regenerates exhibited contractile activity to electrical field stimulation and a qualitatively identical pattern of response to muscarinic, purinergic, alpha- and beta-adrenergic drug administration and nitric oxide. At 4 months after surgery, the maximum forces of contraction of the BAMG regenerates to carbachol stimulation amounted to close to 80 of the host bladder response. With electrical field stimulation, they equaled 44 and 62 of the host bladder response after 2.5 and 4 months, respectively. Histological and immunohistochemical studies confirmed the presence of receptors for neurotransmitters that these functional in vitro studies implied.Conclusions The present study provides further evidence that augmentation cystoplasty with the BAMG leads to functional regeneration of the rat bladder detrusor smooth muscle.
Url:
DOI: 10.1097/00005392-199805000-00100
Affiliations:
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Piechota</name><affiliation wicri:level="2"><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>From the Department of Urology, University of California School of Medicine, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Dahms, Stefan E" sort="Dahms, Stefan E" uniqKey="Dahms S" first="Stefan E." last="Dahms">Stefan E. Dahms</name><affiliation wicri:level="2"><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>From the Department of Urology, University of California School of Medicine, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Nunes, Lora S" sort="Nunes, Lora S" uniqKey="Nunes L" first="Lora S." last="Nunes">Lora S. Nunes</name><affiliation wicri:level="2"><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>From the Department of Urology, University of California School of Medicine, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Dahiya, Rajvir" sort="Dahiya, Rajvir" uniqKey="Dahiya R" first="Rajvir" last="Dahiya">Rajvir Dahiya</name><affiliation wicri:level="2"><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>From the Department of Urology, University of California School of Medicine, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Lue, Tom F" sort="Lue, Tom F" uniqKey="Lue T" first="Tom F." last="Lue">Tom F. Lue</name><affiliation wicri:level="2"><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>From the Department of Urology, University of California School of Medicine, San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Tanagho, Emil A" sort="Tanagho, Emil A" uniqKey="Tanagho E" first="Emil A." last="Tanagho">Emil A. Tanagho</name><affiliation wicri:level="2"><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>From the Department of Urology, University of California School of Medicine, San Francisco</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">The Journal of Urology</title><title level="j" type="abbrev">JURO</title><idno type="ISSN">0022-5347</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">159</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1717">1717</biblScope><biblScope unit="page" to="1724">1724</biblScope></imprint><idno type="ISSN">0022-5347</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-5347</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acellular</term><term>Adrenergic</term><term>Agonist</term><term>Atropine</term><term>Augmentation</term><term>Augmentation cystoplasty</term><term>Bamg</term><term>Biodegradable materials</term><term>Bladder</term><term>Bladder acellular matrix graft</term><term>Bladder reconstruction</term><term>Bladder stone formation</term><term>Bladder strips</term><term>Bladder substitution</term><term>Blood vessels</term><term>Carbachol</term><term>Carbachol stimulation</term><term>Cholinergic</term><term>Collagen content</term><term>Contractile</term><term>Contractile response</term><term>Contractile responses</term><term>Cystoplasty</term><term>Detrusor</term><term>Electrical field stimulation</term><term>Field stimulation</term><term>Functional innervation</term><term>Functional regeneration</term><term>Graft</term><term>Great variety</term><term>Higher dose</term><term>Histological</term><term>Host bladder</term><term>Host bladder response</term><term>Host bladder strips</term><term>Host bladder wall tissue</term><term>Immunohistochemical evaluation</term><term>Individual muscle strip</term><term>Isoproterenol</term><term>Krebs buffer solution</term><term>Lower line</term><term>Matrix</term><term>Maximum forces</term><term>Months postoperatively</term><term>Muscle bundles</term><term>Muscle strips</term><term>Nerve fibers</term><term>Norepinephrine</term><term>Normal micturition</term><term>Oxide pathway</term><term>Partial cystectomy</term><term>Peak contraction values</term><term>Peak contractions</term><term>Pharmacol</term><term>Previous studies</term><term>Protein gene product</term><term>Regenerated</term><term>Regeneration</term><term>Same extent</term><term>Seromuscular colocystoplasty</term><term>Sigma</term><term>Smooth muscle</term><term>Spontaneous muscle activity</term><term>Stock solutions</term><term>Stone formation</term><term>Subsequent administration</term><term>Tion</term><term>Tissue bath</term><term>Tissue bath experiments</term><term>Upper line</term><term>Urinary</term><term>Urinary bladder</term><term>Urinary tract</term><term>Urol</term><term>Urology</term><term>Valethamate</term><term>Valethamate bromide</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Purpose To assess the response of rat urinary bladder regenerated by the homologous bladder acellular matrix graft (BAMG) to in vitro electrical and pharmacologic stimuli.Materials and Methods In Sprague-Dawley rats, partial cystectomy (>50) was performed, followed by BAMG augmentation cystoplasty. After 4 months, organ bath studies of tissue strips in 10 were used to compare the contractility of the BAMG regenerates and the corresponding host detrusor smooth muscle.Results The BAMG regenerates exhibited contractile activity to electrical field stimulation and a qualitatively identical pattern of response to muscarinic, purinergic, alpha- and beta-adrenergic drug administration and nitric oxide. At 4 months after surgery, the maximum forces of contraction of the BAMG regenerates to carbachol stimulation amounted to close to 80 of the host bladder response. With electrical field stimulation, they equaled 44 and 62 of the host bladder response after 2.5 and 4 months, respectively. Histological and immunohistochemical studies confirmed the presence of receptors for neurotransmitters that these functional in vitro studies implied.Conclusions The present study provides further evidence that augmentation cystoplasty with the BAMG leads to functional regeneration of the rat bladder detrusor smooth muscle.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Piechota, Hans J" sort="Piechota, Hans J" uniqKey="Piechota H" first="Hans J." last="Piechota">Hans J. Piechota</name></region><name sortKey="Dahiya, Rajvir" sort="Dahiya, Rajvir" uniqKey="Dahiya R" first="Rajvir" last="Dahiya">Rajvir Dahiya</name><name sortKey="Dahms, Stefan E" sort="Dahms, Stefan E" uniqKey="Dahms S" first="Stefan E." last="Dahms">Stefan E. Dahms</name><name sortKey="Lue, Tom F" sort="Lue, Tom F" uniqKey="Lue T" first="Tom F." last="Lue">Tom F. Lue</name><name sortKey="Nunes, Lora S" sort="Nunes, Lora S" uniqKey="Nunes L" first="Lora S." last="Nunes">Lora S. Nunes</name><name sortKey="Tanagho, Emil A" sort="Tanagho, Emil A" uniqKey="Tanagho E" first="Emil A." last="Tanagho">Emil A. Tanagho</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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